Subconjunctival Injection of Regulatory T Cells Potentiates Corneal Healing Via Orchestrating Inflammation and Tissue Repair After Acute Alkali Burn.
MouseBurnChemical / corrosiveBALB/cMalen = Clinical evaluation combined from 3 independent experiments…Subconjunctival injection (10 microlitres at one site, 33-gauge needle)Tregs suspended in 10 microlitres of PBS per eye (the repor…Immediately after the corneal alkali burn
Model: Unilateral corneal alkali burn of the right eye: 2 mm diameter Whatman III filter paper disk presoaked for 10 s in 1 N NaOH applied to the central cornea for 40 s, followed by 1 min PBS rinsing
Subconjunctival Tregs reached the cornea within 6 h and peaked at 48 h. Treg-treated eyes showed faster epithelial closure (almost complete by 16 h versus about 25% defect remaining in controls), NEI score falling to 0 by day 13 versus a mean of 3 at day 16 in controls, faster resolution of corneal…
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.85. Machine-extracted fields require source verification.