Multi-omics analyses of radiation survivors identify radioprotective microbes and metabolites.
MouseRadiationC57BL/6J, both specific pathogen-free and germ-freeMale and femalen = 57 naive specific pathogen-free mice in the post-radiation…Oral gavage for faecal microbiota transplantation, bacterial strains and tryptophan metabolites; drinking water for short-chain fatty acids; passive transfer by dirty cage sharingFaecal microbiota transplantation 200 microlitres of a susp…Microbiota and bacterial reconstitution completed before radiation, with radiat…
Model: Acute radiation syndrome induced by single total body ionising radiation at 8.0 to 9.2 Gy (dose dependent on sex, age and specific pathogen-free or germ-free status); a separate tumour model used 10 Gy localised radiotherapy to subcutaneous B16F10 melanoma
Elite-survivor microbiota conferred radioprotection: about 75% of dirty cage recipients survived high dose total body radiation versus about 20% of control cage recipients, with lower clinical scores, reduced bone marrow caspase 3 and increased Ki67, and preserved splenic architecture. Lachnospirac…
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