Melatonin promotes regeneration of injured motor axons via MTreceptors.
MixedCrushPeripheral-nerve crushCD1 mice for electrophysiology; C57BL/6 mice expressing cytosolic GFP under a Schwann cell promoter for immunofluorescence; Wistar IGS rats for Schwann cell culturesn = At least n = 4 mice per group for electrophysiology (10 fib…Melatonin intraperitoneal; receptor agonists and antagonists by daily local intramuscular hind-limb injection, luzindole also intraperitoneal in the crush experimentsMelatonin 30 mg/kg in 40 microlitre physiological saline wi…First melatonin injection 30 minutes after crush or transection surgery; daily…
Model: Three models: local hind-limb injection of alpha-latrotoxin (5 microgram/kg) causing reversible presynaptic motor terminal degeneration; sciatic nerve crush (haemostatic forceps for 20 s, 0.5 cm from hip insertion); sciatic nerve transection with edges left juxtaposed
Melatonin accelerated recovery of neurotransmission after alpha-latrotoxin injury and after sciatic crush, with significantly greater compound muscle action potential recovery at 18 and 26 days and complete recovery by 26 days, and increased axonal regrowth after transection with Schwann cell proli…
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