Irisin Pretreatment Protects Kidneys against Acute Kidney Injury Induced by Ischemia/Reperfusion via Upregulating the Expression of Uncoupling Protein 2.
MouseIschaemia-reperfusionRenalC57BL/6, specific pathogen freeMaleIntraperitoneal injection10, 100 and 200 microgram/kg/day, with 100 microgram/kg sel…Pretreatment beginning 14 days before ischaemia-reperfusion surgery
Model: Bilateral renal ischaemia-reperfusion: microaneurysm clamps on both renal pedicles for 45 minutes followed by reperfusion, with blood and kidney harvested 24 hours later. Sham operation without clamping. In vitro: HK-2 hypoxia 3 h (1% O2) then 3 h reoxygenation
Irisin pretreatment reduced serum creatinine, blood urea nitrogen, KIM-1 and tubular injury score, lowered inflammatory markers and adhesion molecules, reduced cleaved caspase-3 and TUNEL-positive apoptosis, and increased UCP2 and phospho-AMPK. Protection was lost with UCP2 knockdown or AMPK inhibi…
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