Pyrophosphate therapy prevents trauma-induced calcification in the mouse model of neurogenic heterotopic ossification.
MouseTBIWeight-drop / impact-accelerationCD1 (main experiments); model also validated in C57BL/6J and Balb/CFemaleIntraperitoneal injection combined with oral administration in drinking water80-100 mg/kg sodium pyrophosphate per intraperitoneal injec…First intraperitoneal dose given immediately (within 1 min) after TBI + CTX or…
Model: Combined polytrauma model of neurogenic heterotopic ossification: weight-drop traumatic brain injury plus cardiotoxin-induced hamstring muscle injury (TBI + CTX); adrenaline plus cardiotoxin (ADR + CTX) used as an alternative model
Combined pyrophosphate treatment starting immediately after injury reduced calcified deposit volume in the hamstring muscle from 5.25 to 0.11 mm3 (p = 0.02), and starting 30 min after injury from 5.25 to 0.08 mm3 (p = 0.03). In the adrenaline plus cardiotoxin model, calcification fell from 3.04 to…
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