An acute immune response underlies the benefit of cardiac stem cell therapy.
MouseIschaemia-reperfusionCardiacC57BL/6J plus Ccr2-RFP and Cx3cr1-GFP reporter lines, Ccr2 and Cx3cr1 gene-deleted mice, mTomato reporter donors and tamoxifen-inducible c-Kit MerCreMer x Rosa26-eGFP lineage-tracing miceMale and femaleIntra-cardiac (intramyocardial) injection under isoflurane anaesthesia via left lateral thoracotomy using a 33-gauge needlePost ischaemia-reperfusion hearts: 150,000 mononuclear cell…One week after ischaemia-reperfusion injury
Model: Myocardial ischaemia-reperfusion injury by temporary left coronary artery ligation with 120 min of ischaemia before reperfusion; permanent left coronary artery occlusion used in a subset of experiments
Mononuclear cells, cardiac progenitor cells, zymosan and freeze-thaw-killed mononuclear cells each significantly improved fractional shortening and left ventricular end-systolic volume 2 weeks after injection compared with saline, with benefit persisting for at least 8 weeks for mononuclear cells a…
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