Mineralocorticoid Receptor Signaling Contributes to Normal Muscle Repair After Acute Injury.
MouseChemical/toxinMineralocorticoid receptor floxed mice on a C57BL6/NCrL background crossed to hemizygous MCK-Cre mice to generate myofiber-specific conditional knockouts and Cre-negative littermate controls; a separate C57BL/10 colony was used for the spironolactone experimentsMale and femalen = Group sizes varied by assay and time point, ranging from n…Oral, administered in water bottlesTreatment initiated 2 weeks prior to barium chloride-induced injury and continu…
Model: Acute skeletal muscle injury by intramuscular injection of 50 microlitres of sterile 1.2% barium chloride into the middle portion of the left tibialis anterior; the right tibialis anterior received 50 microlitres of sterile saline as a contralateral control
CYP11B2 aldosterone synthase was localised in CD11b positive infiltrating myeloid cells of normal skeletal muscle after acute injury, peaking at 4 days. Both the conditional knockout and spironolactone increased the number of IgG-positive damaged myofibers at 4 days post-injury, interpreted as stab…
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