Stabilization of primary cilia reduces abortive cell cycle re-entry to protect injured adult CNS neurons from apoptosis.
RatOtherSprague-Dawley (adult albino; primary cortical neurons from P1 pups)Female for in vivo optic nerve transectionn = n = 3 retinas per group for cilia quantification; n = 4 ret…Intraocular (intravitreal) injection into the vitreous chamber4 microL of tubastatin A at 6.8 microg/microL or Aurora Kin…Intravitreal drug at 3 and 10 days after surgery per the Methods (stated as 3 a…
Model: Optic nerve transection under isoflurane anaesthesia, with the nerve cut within 2 mm of the back of the eye; in vitro models used 5 or 8 hours of hypoxia followed by 24 hours of normoxia, and 50 microM etoposide for 24 hours
Intravitreal tubastatin A and Aurora Kinase A Inhibitor I each significantly increased retinal ganglion cell survival at 14 days after optic nerve transection compared with DMSO, and shRNA knockdown of HEF1, PLK1 or Kif2a improved survival, with NDE1 knockdown improving survival only at middle and…
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