BET protein inhibition regulates cytokine production and promotes neuroprotection after spinal cord injury
MouseSpinal cord injuryContusionC57BL/6Femalen = 72 female C57BL/6 mice in total; RT-qPCR n=24 at 4 h and n=…Intraperitoneal30 mg/kg diluted in 5% DMSO and 5% Tween-80 in salineFirst dose 2 h after the contusion
Model: Moderate contusion spinal cord injury at T11 after laminectomy using the Infinite Horizon impactor at 60 kDyn with tissue displacement of 450-600 um, under ketamine 90 mg/kg and xylazine 10 mg/kg anaesthesia; sham mice underwent the same surgery without contusion
JQ1 significantly reduced IL-6, IL-1beta and TNF-alpha mRNA at 4 h after injury, with IL-1beta still suppressed at 72 h, reduced CCL2 at 72 h and iNOS at 72 h, and increased IL-4 and IL-13 mRNA at 4 h and IL-10 at 72 h, changes corroborated at protein level for IL-10, IL-13 and IL-6. Long-term trea…
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