Film interface for drug testing for delivery to cells in culture and in the brain.
MouseTBIControlled cortical impactC57BL/6 (reported as CL7/BL6 in the retrieved text)n = Necrostatin-1 n = 5 per group; other assays n = 3 to 15silk film placed on the exposed cortical surface within the burr hole, bone flap replaced2 ug (8 nmol) of necrostatin-1 per 4 mm filmfilms implanted 24 h before injury; assessment at 5 h post-injury
Model: Controlled cortical impact over the left parietotemporal cortex, 3 mm flat-tip impounder, 6 m/s, 0.3 mm depth
Film-delivered necrostatin-1 reduced necrotic cell counts by about 40 percent compared with vehicle films (96 +/- 8 versus 164 +/- 8 cells per 200x field), and silk films were well tolerated in brain.
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.85. Machine-extracted fields require source verification.