Insulin ameliorates pulmonary edema through the upregulation of epithelial sodium channel via the PI3K/SGK1 pathway in mice with lipopolysaccharide-induced lung injury.
MouseEndotoxin/LPSC3H/HeNn = 24 mice in four groups, n=6 per groupIntravenous via an internal jugular vein catheter attached to a micro-osmotic pump0.1 U/kg/h at a rate of 2.5 mU/h per mouseStarted 16 h prior to lipopolysaccharide exposure
Model: Lipopolysaccharide-induced acute lung injury, LPS serotype O111:B4 at 5 mg/kg in 50 ul by one-time intratracheal injection, without hyperglycaemia
Insulin reduced lavage protein, cell and neutrophil counts, epithelial permeability, lung injury score, wet to dry ratio and apoptosis and increased alveolar fluid clearance by 93% through increased ENaC expression and SGK1 phosphorylation, without altering blood glucose.
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