Moderate hypothermia inhibits microglial activation after traumatic brain injury by modulating autophagy/apoptosis and the MyD88-dependent TLR4 signaling pathway.
RatTBIFluid percussionSprague-DawleyMalen = 60 rats per group across four groups (240 total), with six…Partial whole-body immersion in an ice-cold water bath under general anaesthesia, with the animal in a plastic bag and head exposedTarget brain temperature 32 degrees C (normothermia control…Started after the fluid percussion injury, with about 30 min needed to reach ta…
Model: Lateral fluid percussion traumatic brain injury, moderate severity 2.1-2.2 atm applied for 10 ms to the exposed dura via a left parietal craniectomy, 24 h after surgical preparation
At 24 h after injury, moderate hypothermia reduced cleaved caspase 3 positive cells (injured cortex 13.3 +/- 1.49 versus 23.2 +/- 2.39) and Iba-1 positive microglia (17 +/- 1.25 versus 22 +/- 1.94), reduced LC3 and Beclin-1 positive microglia, increased cleaved caspase 3 positive microglia (16.9 +/…
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