Biodegradation of ECM hydrogel promotes endogenous brain tissue restoration in a rat model of stroke.
RatIschaemia-reperfusionCerebral (MCAO/stroke)Sprague-DawleyMalen = 56 rats with lesion volume greater than 40 mm3 randomly ass…Intracerebral injection into the stroke lesion cavity using an injection-drainage approach with a 250 uL Hamilton syringe and a 24 G drainage cannula, delivered by frame-mounted injection pump0, 3, 4 and 8 mg/mL protein concentrations; lesion-equivale…14 days after middle cerebral artery occlusion
Model: Transient intraluminal right middle cerebral artery occlusion for 70 minutes followed by reperfusion, with hydrogel implanted into the resulting 14-day-old stroke cavity
Less concentrated hydrogels (3 and 4 mg/mL) degraded by about 95% at 90 days with progressive macrophage infiltration, robust endothelial invasion with neovascularisation, and neural progenitor differentiation into mature neurons plus oligodendrocyte invasion; the stiffer 8 mg/mL hydrogel resorbed…
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