Amelioration of spinal cord injury in rats by blocking peroxynitrite/calpain activity.
RatSpinal cord injuryContusionSprague-DawleyMalen = 21 rats, 7 per group (injury, injury plus GSNO, sham)Intravenous0.05 mg/kg body weight (50 micrograms/kg) in about 25 micro…First dose at 2 h after injury
Model: Contusion spinal cord injury at T9-T10 by computer-controlled impactor, 2 mm tissue deformation, impact velocity 1.5 m/s, contusion time 85 ms; 2-week model
GSNO significantly improved locomotor recovery by day 14 and raised the mechanical withdrawal threshold from day 7 onwards, while significantly reducing 3-nitrotyrosine, nNOS phosphorylation and calpain-derived alpha II spectrin breakdown product 145 kDa relative to untreated injury.
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