Tumor Necrosis Factor Inhibition in the Acute Management of Traumatic Optic Neuropathy.
MouseCrushPeripheral-nerve crushC57BL/6Jn = 40 mice in total, 20 per injury model; 5 mice per treatment…Subcutaneous injection on the dorsum of the neck using a 27-gauge needle10 mg/kg body weightStarted immediately following optic nerve injury
Model: Unilateral optic nerve crush with jeweller's forceps for 10 seconds approximately 1 mm behind the optic disc, and sonication-induced traumatic optic neuropathy (ultrasound shockwave)
Etanercept increased retinal ganglion cell survival relative to vehicle in both models (crush 49 +/- 2 per cent versus 25 +/- 1 per cent of the uninjured fellow eye; sonication 97 +/- 2 per cent versus 77 +/- 2 per cent; both p less than 0.01) and raised pattern electroretinogram a-wave amplitudes…
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