Bioluminescent murine models of bacterial sepsis and scald wound infections for antimicrobial efficacy testing.
MouseBurnScaldBALB/c for the scald wound model and outbred CD1 Swiss for the sepsis modelMalen = Scald model 18 mice in three groups of six; sepsis model tw…Mupirocin topical to the wound; daptomycin intraperitonealMupirocin 200 microlitres of 2% ointment per application, e…Mupirocin first given 24 h after infection, that is day 3 after wounding; dapto…
Model: Partial thickness second degree scald burn of dorsal skin by submersion against an aperture in a 65 degrees C water bath for 45 s then 15 degrees C water for 45 s, infected 2 days later with bioluminescent Staphylococcus aureus Xen29; separate intraperitoneal Staphylococcus aureus Xen29 sepsis model in porcine gastric mucin
Mupirocin significantly reduced photon flux from day 4 and wound area at days 6 and 8 with improved re-epithelialisation, and daptomycin reduced bacterial burden and total flux (p<0.01) with 100% survival and clearance of bacteraemia (log-rank p<0.001).
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