Connexin32 plays a crucial role in ROS-mediated endoplasmic reticulum stress apoptosis signaling pathway in ischemia reperfusion-induced acute kidney injury.
MouseIschaemia-reperfusionRenalC57BL/6, including Cx32 knockout and wild-type littermates on a C57BL/6 backgroundmalen = n=6 per groupIntraperitoneal4-PBA 100 mg/kg; TUDCA 300 mg/kg; N-acetylcysteine 200 mg/k…1 h before renal ischaemia (pretreatment)
Model: Renal ischaemia-reperfusion by bilateral renal pedicle occlusion with an atraumatic microvascular clamp for 45 min followed by reperfusion; body temperature maintained at 37 degrees C; reperfusion assessed at 8, 12, 16, 24 and 48 h, with the main studies at 24 h
4-PBA and TUDCA attenuated tubular epithelial apoptosis, histological damage and renal functional impairment after ischaemia-reperfusion, and N-acetylcysteine reduced GRP78, XBP1 and CHOP expression. Cx32 deletion reduced ROS spread and TUNEL-positive cells compared with wild-type littermates.
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.9. Machine-extracted fields require source verification.