Celastrol treatment protects against acute ischemic stroke-induced brain injury by promoting an IL-33/ST2 axis-mediated microglia/macrophage M2 polarization.
RatIschaemia-reperfusionCerebral (MCAO/stroke)Sprague-DawleyMalen = 36 rats in total, allocated to vehicle (12 rats) or celastr…Intraperitoneal injection1 mg/kg dissolved in 0.9% NaCl with 1% dimethyl sulfoxide;…Immediately after occlusion and again 1 day later (0 and 24 hours)
Model: Modified middle cerebral artery occlusion producing permanent focal ischaemia using a monofilament nylon suture of 0.24 to 0.26 mm diameter advanced into the internal carotid artery, with sham surgery lacking filament insertion
Celastrol reduced infarct volume from about 40% to about 20%, reduced neuronal apoptosis, improved rotarod and adhesive removal performance, and raised IL-33 and IL-10 while lowering IL-1beta, IL-6 and TNF-alpha relative to occlusion alone.
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.9. Machine-extracted fields require source verification.