Effects of nicotine on corneal wound healing following acute alkali burn.
MouseChemical/toxinBALB/cFemalen = 6 to 7 mice per group; total not statedOral, in drinking water100 micrograms/ml or 200 micrograms/ml in 2% saccharin drin…Started 1 week before the alkali burn
Model: Corneal alkali burn produced by placing 2 mm diameter Whatman #3 filter paper soaked in 1N NaOH on the central cornea for 40 seconds after topical 0.5% proparacaine hydrochloride, followed by rinsing with 25 ml of phosphate buffered saline, under Zoletil anaesthesia at 0.2 ml/kg intramuscularly
Chronic oral nicotine dose-dependently increased corneal neovascularisation at days 7 and 14 and upregulated VEGF, MMP9, alpha-SMA, TGF-beta and Col1 expression relative to vehicle, indicating accelerated angiogenic and fibrogenic responses after alkali injury.
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