Involvement of p38-betaTrCP-Tristetraprolin-TNFalpha axis in radiation pneumonitis.
MixedRadiationTtp-null and wild-type on C57BL/6 background; separate C57BL/6 versus C3H comparisonMalen = n=6 mice per group per time point per genotype for TNF-alph…Added to cell culture medium; no in vivo administration of SB203580 reportedSB203580 1 micromolar in vitro; beta-TrCP1 siRNA 10 and 25…30 min before irradiation, or 1 h before irradiation for the clonogenic assay
Model: Single-dose thoracic irradiation of 15 Gy to the lungs; in vitro irradiation at 4 Gy using a Philips RT250 orthovoltage source at 250 kV
Ttp-null mice showed elevated basal (9.54 plus or minus 0.67 fold) and post-irradiation TNF-alpha (12.2 plus or minus 0.85 fold, p<0.02) with sustained acute lung inflammation, and SB203580 blocked radiation-induced TTP degradation and radioprotected lung macrophages (enhancement ratio 0.86) withou…
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