Notch-Expanded Murine Hematopoietic Stem and Progenitor Cells Mitigate Death from Lethal Radiation and Convey Immune Tolerance in Mismatched Recipients
MouseRadiationBALB/cJ recipients; B6-Ly5a donors; C3H third-party skin graft donorsFemale recipients (donor B6-Ly5a mice were female or male)n = n = 12 per group for fresh cells; n = 10 to 15 for the 1 an…Intravenous1, 3, 5 or 15 x 10^6 expanded LSK cells per mouse; 5 x 10^6…2 to 4 hours after total body irradiation in the main experiments, with delayed…
Model: Haematopoietic acute radiation syndrome induced by a single dose of 6.5 to 8.5 Gy total body gamma irradiation from a caesium source at 81.4 cGy/min
Delta1-expanded cells gave high level donor engraftment by day 7 and improved survival after lethal total body irradiation in a cell dose-dependent manner (median survival 24 versus 14 days for fresh cells after 8.5 Gy). Cryopreserved cells at 5 x 10^6 given 2 to 4 hours after 7.5 to 8.5 Gy markedl…
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