Modeling Acinetobacter baumannii wound infections: The critical role of iron.
MouseCrushLimb / muscle crushC57BL/6Malen = 15 per group in the injury model experiments; 30 per group…Topical wound washout200 microlitres of 100 mM FeCl3 in 0.9% normal salineImmediately after bacterial inoculation, as the washout solution before skin cl…
Model: Rectus abdominis muscle crush injury with forceps combined with ischaemia produced by double suture ligation of the muscle including the epigastric vascular bundle, followed by inoculation of the wound with 100 microlitres of Acinetobacter baumannii ATCC 19606 suspension for 2 minutes
Combined crush injury, ischaemia and inoculation produced clinical wound infection in 100% of mice, with the highest bacterial burden (about 10^5 CFU/mg) and an approximately threefold increase in HIF1-alpha. Iron washout of the wound prevented wound infection entirely (n = 30 per group, p < 0.0001…
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