Inhibition of Na-K-Cl cotransporter isoform 1 reduces lung injury induced by ischemia-reperfusion.
MouseIschaemia-reperfusionOther / unspecifiedWild-type, SPAK knockout and WNK4 knockinMalen = n = 6 per groupAdministered via the lung perfusateDuring reperfusion
Model: Isolated in situ perfused mouse lung with main pulmonary artery and left atrium cannulated and perfusate of 1 mL blood plus 9 mL physiological solution; 120 min ischaemia with no ventilation or perfusion followed by 60 min reperfusion
In wild-type mice, ischaemia-reperfusion caused oedema (wet/dry 6.30 plus or minus 0.36), hyperpermeability (0.29 plus or minus 0.04), neutrophil sequestration (255.0 plus or minus 55.8 cells per high-power field) and nuclear factor-kappa B activation. Injury was more severe in WNK4 knockin and att…
Extraction: extract · Supplied extraction confidence: 0.75. Machine-extracted fields require source verification.