The cross-talk between autophagy and endoplasmic reticulum stress in blood-spinal cord barrier disruption after spinal cord injury.
RatSpinal cord injuryCompressionSprague-DawleyFemalen = Evans blue n = 4, western blot n at least 3, immunofluoresc…Intraperitoneal injection; Evans blue given intravenously4-phenylbutyric acid 100 mg/kg and chloroquine 50 mg/kg in…Immediately after spinal cord injury
Model: T9 laminectomy followed by clip-compression crush of the spinal cord with a vascular clip at 30 g force for 2 minutes under chloral hydrate anaesthesia; sham had identical surgery without compression
4-phenylbutyric acid reduced barrier permeability, preserved tight and adherens junction proteins and lowered endoplasmic reticulum stress and autophagy markers, whereas chloroquine worsened permeability and junction protein loss and abolished the benefit of 4-phenylbutyric acid when co-administere…
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