shRNA mediated knockdown of Nav1.7 in rat dorsal root ganglion attenuates pain following burn injury.
RatBurnContact / flameSprague-DawleyMalen = 68 adult male Sprague-Dawley rats across four groups; 16 pe…Dorsal root ganglion microinjection (L4, described as L5 in the conclusions)4 microlitres of vector; titre not statedDay zero, the same day as the burn injury
Model: Focal second-degree contact burn, hind paw held for 15 seconds on a metal plate heated by circulating water at 85 degrees Celsius under isoflurane anaesthesia
Burn injury lowered mechanical and heat withdrawal thresholds for 14 to 21 days and increased dorsal root ganglion Nav1.7 and c-fos at day 7. Nav1.7 shRNA delivered on day zero attenuated mechanical allodynia from day 3 (p = 0.0008) and thermal hyperalgesia from day 7 (p < 0.0001), reduced dorsal r…
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