Transplantation of Mesenchymal Stem Cells for Acute Spinal Cord Injury in Rats: Comparative Study between Intralesional Injection and Scaffold Based Transplantation.
RatSpinal cord injuryContusionSprague-DawleyMalen = 48 rats, 12 per group (4 for histology and 8 for biochemica…Intralesional injection into the injured cord with a 26-gauge Hamilton needle, or scaffold applied to the dorsal surface of the injured cord10 microlitre cell suspension per animal for intralesional…Immediately after the contusive injury (acute spinal cord injury)
Model: Contusive spinal cord injury at T9 after T8-10 laminectomy, 25 g-cm contusion with the MASCIS impactor (10 g rod dropped from 2.5 cm)
Scaffold-based delivery gave higher mesenchymal stem cell engraftment (25.6 plus or minus 1.7 for PLGA and 26.7 plus or minus 1.8 for chitosan versus 20.6 plus or minus 0.7 cells per high power field for intralesional injection) and favoured oligodendrocytic over astrocytic differentiation. BDNF an…
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