Intraspinal transplantation of subventricular zone-derived neural progenitor cells improves phrenic motor output after high cervical spinal cord injury.
RatSpinal cord injuryHemisection / partialSprague-Dawley (adult recipients); neonatal EGFP-transgenic Sprague-Dawley SD-Tg(GFP)2BalRrrc donorsFemalen = Cohort 1: 8 NPC transplant, 4 sham transplant, 4 C2Hx only.…Intraspinal injection at C2-3, ventromedial white matter approximately 1 mm caudal to the lesion and 1.5 mm below the dorsal surface, via 10 microlitre Hamilton syringe and 31 gauge needleApproximately 500,000 live cells in 5 microlitres of growth…7 days after C2 hemisection
Model: C2 spinal cord hemisection (C2Hx), high cervical spinal cord injury
Transplantation increased inspiratory tidal volume during hypoxic (P=0.003) and hypercapnic (P=0.019) challenge at 4 and 8 weeks, and increased ipsilateral phrenic burst amplitude across conditions (P<0.001), with no change in contralateral phrenic output, burst frequency or eupneic breathing. Roug…
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