Intracerebroventricular administration of chondroitinase ABC reduces acute edema after traumatic brain injury in mice.
MouseTBIControlled cortical impactC57BL/6Malen = 40 mice in total, n = 10 per groupIntracerebroventricular injection into the right lateral ventricle, needle to 3 mm depth at 1 mm right and 0.3 mm anterior to bregma10 microlitres of filter-sterilised 50 U/ml chondroitinase…Within 5 min after injury
Model: Controlled cortical impact through a 5 mm craniotomy, 3 mm flat cylindrical indenter driven to 1.3 mm depth at 5 m/s with 300 ms dwell at 10 degrees to the vertical
Injury raised ipsilateral water fraction by 1.13 per cent relative to the contralateral hemisphere; treatment reduced ipsilateral water fraction by 0.54 per cent (p < 0.05), eliminating almost half the injury-induced oedema, with no significant effect in uninjured animals.
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