Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves brain ischemia-induced pulmonary injury in rats associated to TNF-alpha expression.
RatIschaemia-reperfusionCerebral (MCAO/stroke)Sprague-DawleyMalen = 45 rats, 15 per group across three groupsIntravenous, via tail vein over 10 min2 ml of cell suspension prepared at 1 x 10^6 cells per ml (…1 day after middle cerebral artery occlusion
Model: Permanent focal cerebral infarction by bipolar coagulation of the left middle cerebral artery, producing secondary acute lung injury
Transplanted cells survived and distributed widely in lung. At 7 days the wet-to-dry weight ratio fell, arterial oxygen tension rose and carbon dioxide tension fell relative to brain ischaemia alone (all P<0.05), with reduced TNF-alpha mRNA and protein at 3 and 7 days and fewer inflammatory cells.
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.85. Machine-extracted fields require source verification.