Methylene blue exerts a neuroprotective effect against traumatic brain injury by promoting autophagy and inhibiting microglial activation.
MouseTBIControlled cortical impactInstitute for Cancer Research (ICR)malen = 210 mice in total, 70 per groupintraperitoneal1 mg/kg at 1 h after injury, 0.5 mg/kg at 6 h after injury,…first dose infused 1 h after injury
Model: Controlled cortical impact to the right frontoparietal cortex (3.0 m/s, 100 ms dwell, 2 mm depth, 2.5 mm tip) after 4 mm craniotomy
Methylene blue reduced injured hemisphere brain water content at 24 h, lowered modified neurological severity scores at 24 and 72 h, reduced lesion volume at all time points, preserved neurons and inhibited microglial activation at 72 h and 14 days, with increased markers of autophagy.
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.92. Machine-extracted fields require source verification.