HMGB1 promotes the activation of NLRP3 and caspase-8 inflammasomes via NF-kappaB pathway in acute glaucoma
MouseIschaemia-reperfusionOther / unspecifiedC57BL/6FemaleIntravitreal injection120 micromol/L in 2 microlitresImmediately after induction of ischaemia-reperfusion injury
Model: Acute glaucoma modelled by anterior chamber cannulation of the right eye with a 30-gauge needle and Balanced Salt Solution infusion raising intraocular pressure to 70 mmHg for 60 min, producing retinal ischaemia-reperfusion injury and retinal ganglion cell death; left eye served as control
HMGB1 rose in ischaemic retina from 6 h and peaked at 48 h. Exogenous recombinant HMGB1 worsened retinal thinning and retinal ganglion cell loss and increased cleaved caspase-8, NLRP3, ASC, caspase-1 processing, phospho-NF-kappaB p65 and IL-1beta, whereas the HMGB1 inhibitor glycyrrhizic acid reduc…
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