Silencing the alpha2 subunit of gamma-aminobutyric acid type A receptors in rat dorsal root ganglia reveals its major role in antinociception posttraumatic nerve injury.
RatCrushPeripheral-nerve crushSprague-DawleyFemale (retired breeders)n = 4 to 10 rats per behavioural group; 6 to 14 rats per group…Direct application onto the right L5 dorsal root ganglionNO-711 50 micrograms in 30 microlitres; oligodeoxynucleotid…Postoperative day 2, after thermal and mechanical hypersensitivity had been con…
Model: Right sciatic nerve crush injury (single 30 s crush with a serrated haemostat approximately 7 mm from nerve emergence)
Crush injury reduced ipsilateral L5 dorsal root ganglion alpha2 subunit expression by about 30% at day 2 and about 35% at day 10. Antisense knockdown worsened thermal and mechanical hypersensitivity in injured rats (days 3 to 6) and produced a neuropathic-like phenotype in sham animals, with an app…
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