Can Cytoprotective Cobalt Protoporphyrin Protect Skeletal Muscle and Muscle-derived Stem Cells From Ischemic Injury?
MouseIschaemia-reperfusionLimb / skeletal muscleC57BL/6n = 7 mice per group in three post-injury treatment groups, plu…Intraperitoneal (in vivo); 25 microM single exposure in vitroAdministered during ischaemia, at the onset of reperfusion, or 1 h after reperf…
Model: Unilateral hindlimb ischaemia produced by 2 h tourniquet application followed by 24 h reperfusion; parallel in vitro hypoxia (5 h, <0.5% O2) and 24 h reoxygenation of muscle-derived stem cells
CoPP given during ischaemia reduced gastrocnemius myocyte damage by 38% (86.4% to 53.2% Evans blue positive fibres, p<0.001) and by 30% when given at reperfusion onset; tibialis anterior damage fell 27% only with dosing at reperfusion onset. Delaying to 1 h after tourniquet release abolished protec…
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