Sponge-mediated lentivirus delivery to acute and chronic spinal cord injuries.
MouseSpinal cord injuryHemisection / partialC57BL/6Femalen = 4 to 5 mice per condition, with 5 per group for immunohisto…Intrathecal, by sponge implantation into the intrathecal space without entering the cordLentivirus pipetted onto the macroporous sponge immediately…Acute delivery immediately after injury, or chronic delivery 4 weeks after inju…
Model: Dorsal laminectomy at T9 to T10 with a 2.25 mm lateral hemisection and insertion of a seven-channelled microporous poly(lactide-co-glycolide) bridge
Intrathecal sponge-mediated lentivirus gave transgene expression persisting over 12 weeks without detectable cord damage, with two-week expression about three-fold higher in acutely injured than uninjured cords. Shh lentivirus placed directly above the lesion reduced Msx2 reporter activity, increas…
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