Radiation exposure induces inflammasome pathway activation in immune cells.
MouseRadiationCD-1 outbred for dose and kinetic studies; caspase 1 knockout (NOD.129S2(B6)-Casp1/LtJ) with NOD/ShiLtJ controls; Nlrp3 knockout on C57BL/6J with C57BL/6J controlsMaleIntraperitoneal20 mg/kg2 h before and 6 h after radiation exposure
Model: Whole-body caesium-137 irradiation at 0.5, 1, 2 or 4 Gy, dose rate 1.63 to 1.65 Gy per minute
Radiation caused dose-dependent, delayed inflammasome activation detectable from day 1 and sustained to day 7, returning to baseline by day 14. Allopurinol at 2 Gy significantly reduced caspase 1 activation in macrophages, dendritic cells and NK cells to sham levels and suppressed LPS-stimulated IL…
Extraction: extract · Supplied extraction confidence: 0.85. Machine-extracted fields require source verification.