Induced pluripotent stem cell therapy ameliorates hyperoxia-augmented ventilator-induced lung injury through suppressing the Src pathway.
MouseOtherC57BL/6 wild type; Src-deficient heterozygotes on C57BL/6 background; iPSCs reprogrammed from mouse embryonic fibroblasts with Oct4, Sox2 and Parp1Malen = n=5 per group for most assays across six groups; n=3 per gr…Intravenous, tail vein5 x 10^7 cells/kgOne hour before mechanical ventilation
Model: Hyperoxia-augmented ventilator-induced lung injury using high tidal volume ventilation at 30 mL/kg with room air or hyperoxia for 1 to 4 h; non-ventilated mice as controls
Intravenous induced pluripotent stem cells given one hour before ventilation reduced Src phosphorylation, oxidative stress, neutrophil influx, MIP-2 and PAI-1, microvascular leak, oedema, epithelial apoptosis and lung injury score while improving oxygenation, mirroring protection in Src-deficient m…
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