Interleukin-6, but not the interleukin-6 receptor plays a role in recovery from dextran sodium sulfate-induced colitis.
MouseChemical/toxinC57BL/6 background; Il-6r-deficient (CMVCre), myeloid-specific LysMCre/Il-6r-floxed and littermate controlsMale for the IL-6 monoclonal antibody experiment and the LysMCre acute and chronic studies; female for the global Il-6r-deficient acute studyn = 4-16 animals per group; severe acute DSS subgroup 9 LysMCre…Intraperitoneal250 micrograms per mouse per injectionFirst dose 1 h before replacing drinking water with 1.5% DSS (day 0)
Model: Dextran sodium sulfate (DSS) induced colitis, acute (1.5-3% DSS in drinking water for 5 days followed by 5 days water) and chronic (four cycles of 1.5%, 1.0%, 1.25% and 1.5% DSS)
IL-6 monoclonal antibody treatment produced a small but statistically significant improvement on day 10 only, with body weight 97% of baseline versus 92% in controls and clinical score 1 versus 3. Global Il-6r-deficient and myeloid-specific LysMCre/Il-6r-deficient mice were indistinguishable from c…
Extraction: extract · Supplied extraction confidence: 0.9. Machine-extracted fields require source verification.