Human CD34+ progenitor cells freshly isolated from umbilical cord blood attenuate inflammatory lung injury following LPS challenge.
MouseEndotoxin/LPSNOD.CB17-Prkdc scid (SCID)Malen = n=4 per group for permeability, myeloperoxidase, neutrophil…Retro-orbital intravenous injection0.2 x 10^6 cells per mouse in 150 microlitres3 hours after lipopolysaccharide challenge
Model: Lipopolysaccharide (Escherichia coli O111:B4) 5 mg/kg body weight by intraperitoneal injection; the survival study used a lethal dose of 11 mg/kg
CD34+ cells reduced lung vascular permeability, myeloperoxidase activity, neutrophil infiltration and pro-inflammatory gene expression, increased endothelial proliferation and improved survival, with 40 per cent surviving at least one week whereas all phosphate-buffered saline and CD34-negative tre…
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