Complement mediates a primed inflammatory response after traumatic lung injury.
MouseOtherC57BL/6 wild type, C3-deficient and C5-deficient miceMaleIntraperitoneal, with anti-C5a also given intravenously in some experimentsAnti-C5a antibody 45 micrograms; C5a receptor antagonist 2…Anti-C5a 20 minutes before injury; hirudin 1 hour before and 30 minutes after i…
Model: Blunt chest trauma producing pulmonary contusion, delivered with a cortical contusion impactor to the right chest along the posterior axillary line 1 cm above the costal margin; a second hit of 50 micrograms intratracheal lipopolysaccharide (O111:B4) was given 24 hours after injury
C5a deficiency or anti-C5a before injury reduced lavage neutrophils, serum CXCL1 and IL-6 and histopathology; hirudin lowered lavage thrombin, C5a, neutrophils and CXCL1 although IL-6 and IL-10 were unchanged; anti-C5a or C5a receptor antagonism before the lipopolysaccharide second hit abolished th…
Extraction: extract · Supplied extraction confidence: 0.85. Machine-extracted fields require source verification.