Plasminogen initiates and potentiates the healing of acute and chronic tympanic membrane perforations in mice.
MouseWoundC57BL/6 background, plasminogen-deficient (plg-/-) and wild-type littermatesn = plg-/- local dose groups n=10 each plus n=10 wild-type; wil…Local injection into the soft tissue at the internal end of the external ear canal; also subcutaneous and intraperitonealLocal: 0.4, 0.2 or 0.1 mg per day in 20 microlitres. Subcut…Acute model from day 0 (day of perforation); chronic model from day 10, after 9…
Model: Standardised perforation of the pars tensa in the posterior superior quadrant with a myringotomy lancet under otomicroscopy; chronic-like perforations produced by leaving plg-/- perforations untreated for 9 days
Local plasminogen restored healing in plg-/- mice dose dependently, with closure at day 9 in 10 of 10 mice at 0.4 and 0.2 mg per day, 6 of 10 at 0.1 mg per day and 0 of 10 with PBS (P<0.0001). In wild-type mice, day 7 healing was 13 of 21 with plasminogen versus 1 of 12 with PBS (P=0.0027). Subcuta…
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