Delineating the relationships among the formation of reactive oxygen species, cell membrane instability and innate autoimmunity in intestinal reperfusion injury.
MouseIschaemia-reperfusionIntestinal / mesentericWild-type C57BL/6J, with a separate arm using SOD1-overexpressing transgenic miceMalen = Trehalose n=11 versus saline n=11; malondialdehyde n=5 per…Intraperitoneal1 g/kg, dissolved in normal saline at 100 mg/ml30 minutes before induction of intestinal ischaemia
Model: Laparotomy with a microclip (125 g pressure) on the superior mesenteric artery, 40 minutes of ischaemia followed by 3 hours of reperfusion; sham animals underwent laparotomy without clipping
Trehalose reduced the mucosal pathology score from 25 plus or minus 4 with saline to 10 plus or minus 2 (p<0.01) and abolished villus IgM and C3 deposition, while lipid peroxidation was unchanged (malondialdehyde 1.8 plus or minus 0.21 versus 1.99 plus or minus 0.31 micromolar; 4-HNE positive villi…
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