Supplementation with gamma-tocopherol attenuates endotoxin-induced airway neutrophil and mucous cell responses in rats.
RatEndotoxin/LPSF344/N (Fisher 344)Malen = n=7 per group across 6 groups, with a separate n=3 per grou…Oral gavage30 mg/kg body weight per day in 0.5 ml of tocopherol-stripp…Started 2 days before intranasal lipopolysaccharide, so two doses preceded the…
Model: Intranasal instillation of lipopolysaccharide in pyrogen-free saline, 150 microlitres into each nasal passage (300 microlitres total) at a total dose of 0, 5 or 20 micrograms (2500 and 10,000 endotoxin units) under light isoflurane anaesthesia, repeated 24 hours later, with tissues collected 24 hours after the last dose
Gamma-tocopherol raised plasma vitamin E four to five fold to about 1.5 micromolar and returned lavage neutrophils, macrophages, protein, prostaglandin E2 and secreted mucins towards control values, normalised the 230 and 900 per cent increases in proximal and distal airway intraepithelial mucosubs…
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