Inhibition of NADPH oxidase activation in oligodendrocytes reduces cytotoxicity following trauma.
MouseSpinal cord injuryContusionWild-type littermates of GFAP-IkappaBalpha-dn transgenic mice; gp91 phox deficient B6.129S6-Cybb/J mice used for in vitro oligodendrocyte isolationFemalen = n=11-12 per group for Basso Mouse Scale; n=7-12 for grid wa…IntraperitonealApocynin 10 mg/kg in vivo (10, 100 or 500 microM in vitro);…First dose 1 h after spinal cord injury; in vitro apocynin pre-treatment 30 min…
Model: Moderate to severe contusion injury at T9 using the Ohio State University electromagnetic spinal cord injury device (0.5-0.6 cm displacement) after T8-T10 laminectomy; in vitro fluid percussion injury of astrocyte cultures (5 atmospheres, 25 ms, applied twice)
In vitro, apocynin at 10 and 100 microM reduced injured astrocyte media induced oligodendrocyte death from 61.5 +/- 6.02 per cent to 28.3 +/- 4.2 and 34.3 +/- 4.8 per cent, while 500 microM was ineffective; gp91 phox deficient oligodendrocytes were protected from injured astrocyte media and from 13…
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