Acrolein involvement in sensory and behavioral hypersensitivity following spinal cord injury in the rat
RatSpinal cord injuryContusionSprague-DawleyMalen = n = 4 per group for TRPA1 quantitative PCR; large diameter…Intraperitoneal5 mg/kgTwo regimens, dosing started immediately after spinal cord injury, or delayed s…
Model: T10 contusion spinal cord injury by weight drop (NYU impactor, 10 g weight dropped 25 mm onto intact dura) after T10 laminectomy; a separate experiment used intraspinal microinjection of acrolein 1.6 microlitres into dorsal horn of uninjured rats
Spinal cord injury raised acrolein-lysine adducts at 1 day (39.43 +/- 2.46 arbitrary units) and 2 weeks (30.38 +/- 2.71) versus sham (9.68 +/- 4.67, P < 0.001), doubled dorsal root ganglion TRPA1 mRNA (2.03 +/- 0.11 fold), increased the proportion of acrolein responsive neurons from 38 to 58 percen…
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.88. Machine-extracted fields require source verification.