Time-dependent changes in the microenvironment of injured spinal cord affects the therapeutic potential of neural stem cell transplantation for spinal cord injury.
MouseSpinal cord injuryContusionC57BL/6J host; donor neural stem/progenitor cells from ffLuc-cp156 transgenic mice on a C57BL/6J background, embryonic day 14 striataFemalen = 52 mice in total; 10 per group for the sub-acute and chroni…Intraparenchymal injection into the lesion epicentre at 1 microlitre/minCell suspension in 2 microlitres; exact cell number not rec…9 days post injury (sub-acute group) or 42 days post injury (chronic group)
Model: Contusive spinal cord injury at T10 after laminectomy using an Infinite Horizon impactor at a force-defined impact of 60 kdyn
Grafts at 9 days post injury improved BMS score, stride length, Rotarod performance, motor evoked potentials and tissue sparing, whereas identical grafts at 42 days gave no benefit despite equivalent survival and differentiation, with graft spread restricted by CS56-positive glial scar.
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