5-AED enhances survival of irradiated mice in a G-CSF-dependent manner, stimulates innate immune cell function, reduces radiation-induced DNA damage and induces genes that modulate cell cycle progression and apoptosis.

PMID 22843381 ↗2012Reported benefit

5-AED enhances survival of irradiated mice in a G-CSF-dependent manner, stimulates innate immune cell function, reduces radiation-induced DNA damage and induces genes that modulate cell cycle progression and apoptosis.

MouseRadiationCD2F1 (antibody blocking and survival studies); C3H/HeN (phagocytosis, oxidative burst and PCR studies); C3H/FeJ (halo-comet assays)Malen = Neutralising antibody experiments repeated twice with n = 1…Subcutaneous injection30 mg/kg s.c. for gene expression experiments; 10, 30 and 1…24 h before irradiation (pre-treatment); neutralising antibodies to G-CSF or IL…

Model: Acute radiation syndrome after total body irradiation: 9.25 Gy gamma at 0.6 Gy/min (survival), 3 Gy gamma (innate immune cell function), 7.5 Gy gamma (gene expression), and 1, 3 or 5 Gy X-rays at 2 Gy/min (comet assays)

After 9.25 Gy TBI, 30-day survival was 100% with 5-AED plus isotype control antibody versus 44% with vehicle plus isotype control; neutralising anti-G-CSF abolished the benefit (31% survival) whereas anti-IL-6 did not (100% survival), showing G-CSF dependence. 5-AED increased phagocytosis in circul…

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5-AED enhances survival of irradiated mice in a G-CSF-dependent manner, stimulates innate immune cell function, reduces radiation-induced DNA damage and induces genes that modulate cell cycle progression and apoptosis.

Evidence by therapy association

5-AED enhances survival of irradiated mice in a G-CSF-dependent manner, stimulates innate immune cell function, reduces radiation-induced DNA damage and induces genes that modulate cell cycle progression and apoptosis.