Heparin disrupts the CXCR4/SDF-1 axis and impairs the functional capacity of bone marrow-derived mononuclear cells used for cardiovascular repair.
MouseWoundin vitro pretreatment of the cells, which were then injected in the mouse ear-wound homing modelheparin 20 U/mL for the in vitro incubation experiments; bi…cells pretreated before injection
Model: mouse ear-wound model used to assess homing of injected bone marrow-derived mononuclear cells
Heparin dose-dependently inhibited basal and SDF-1-induced migration, abrogated SDF-1-induced invasion (16 plus or minus 8 percent of control, P < 0.01) and reduced in vivo homing of injected cells in the mouse ear-wound model (69 plus or minus 10 percent of control, P < 0.05), without affecting ap…
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