Proximal tubule sphingosine kinase-1 has a critical role in A1 adenosine receptor-mediated renal protection from ischemia.
MouseIschaemia-reperfusionRenalC57BL/6 wild type, plus sphingosine kinase-1 and sphingosine kinase-2 knockout, S1P1R floxed and PEPCK-Cre transgenic linesMalen = n=3 per sham group; n=4 to 6 per treatment group in the MK5…Intraperitoneal0.1 mg/kg15 min before renal ischaemia
Model: Renal ischemia-reperfusion injury (30 min renal ischaemia, 24 h reperfusion, with right nephrectomy)
CCPA reduced plasma creatinine, tubular necrosis and renal injury score in wild type and sphingosine kinase-2 knockout mice but not in sphingosine kinase-1 knockout mice. Protection was abolished by the S1P1R antagonist W146, by S1P1R siRNA, by proximal tubule specific S1P1R deletion, by ABCC1 inhi…
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.92. Machine-extracted fields require source verification.