Electroporation-mediated in vivo gene delivery of the Na+/K+-ATPase pump reduced lung injury in a mouse model of lung contusion.
MouseCrushOther / unspecifiedC57BL/6malen = n=9 per group at each of 24, 48 and 72 h plus 9 naive contr…intratracheal instillation followed by transthoracic electroporation100 micrograms plasmid DNA in 100 mM NaCl; electroporation…within several minutes after lung contusion, under the same anaesthetic
Model: unilateral lung contusion produced by a cortical contusion impactor striking the right chest 1 cm above the costal margin along the posterior axillary line (piston velocity 5.8 m/s, depth 10 mm)
Gene delivery of the Na+/K+-ATPase subunits immediately after contusion reduced BAL albumin, improved compliance and hysteresis at 24, 48 and 72 h and lessened alveolar inflammation on histology.
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