Assessment of Glial Scar, Tissue Sparing, Behavioral Recovery and Axonal Regeneration following Acute Transplantation of Genetically Modified Human Umbilical Cord Blood Cells in a Rat Model of Spinal Cord Contusion.
RatSpinal cord injuryContusionWistarMale and femalen = 43 rats total: n=12 VEGF+GDNF, n=10 EGFP, n=11 SCI only, n=…Intraspinal injection at two points 1 mm rostral and caudal to the injury centre2 x 10^6 cells per rat given as two intraspinal injections…Immediately after contusion or laminectomy
Model: Weight-drop contusion at Th8 (2 mm rod, 10 g, dropped from 25 mm) after laminectomy
At day 30 the VEGF+GDNF group had greater intact tissue (53.1 +/- 4.5 per cent) than EGFP cells (47 +/- 1.8) or untreated SCI (38.8 +/- 1.2), smaller cavity volume (7.4 +/- 1.3 versus 11.7 +/- 2.5 and 31.9 +/- 2.1 per cent), about three-fold lower GFAP, and higher BBB scores at day 29 (16.14 +/- 2…
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